NG2 antibody [7G4E5]

Cat. No. GTX60751

Host	Mouse
Clonality	Monoclonal
Clone Name	7G4E5
Isotype	IgG1
Application	WB, FACS, ELISA
Reactivity	Human
Package	100 μg ($399)

See all NG2 products

Datasheet
Document

View product citations for antibody GTX60751 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

251 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NG2 (AA: 2247-2308) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chondroitin sulfate proteoglycan 4 , HMW-MAA , MCSP , MCSPG , MEL-CSPG , MSK16 , NG2

Cellular Localization

Cell membrane,Apical cell membrane

Background

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1464 CSPG4
UniProt: Q6UVK1 CSPG4

Research Area

DATA IMAGES

GTX60751 ELISA Image

ELISA analysis of antigen using GTX60751 NG2 antibody [7G4E5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60751 WB Image

WB analysis of HEK293 (1) and NG2(AA: 2247-2308)-hIgGFc transfected HEK293 (2) cell lysate using GTX60751 NG2 antibody [7G4E5].

GTX60751 FACS Image

FACS analysis of HEK293 cells using GTX60751 NG2 antibody [7G4E5].
Green : NG2
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty