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NG2 antibody

Anti-NG2 antibody used in Western Blot (WB). GTX03452
Anti-NG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03452
Anti-NG2 antibody used in Western Blot (WB). GTX03452
Anti-NG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03452
Anti-NG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03452

Cat. No. GTX03452

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

251 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human CSPG4(Accession Q6UVK1), corresponding to amino acid residues A1854-R1874.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chondroitin sulfate proteoglycan 4 , HMW-MAA , MCSP , MCSPG , MEL-CSPG , MSK16 , NG2

Cellular Localization

Cell membrane , Apical cell membrane

Background

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NG2 antibody used in Western Blot (WB). GTX03452

GTX03452 WB Image

WB analysis of mouse lung tissue lysate using GTX03452 NG2 antibody. The lane on the left was treated with blocking peptide.

Anti-NG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03452

GTX03452 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX03452 NG2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-NG2 antibody used in Western Blot (WB). GTX03452

GTX03452 WB Image

WB analysis of A375 whole cell lysate using GTX03452 NG2 antibody. The lane on the left was treated with blocking peptide.

Anti-NG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03452

GTX03452 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX03452 NG2 antibody.
Permeabilization : 0.1% Triton X-100
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Dilution : 1:200

Anti-NG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03452

GTX03452 IHC-P Image

IHC-P analysis of mouse pancreatic tissue using GTX03452 NG2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399