*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
May be used in monitoring for NGF (2.5S, β and 7S).
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NGF-2.5S from male mouse submaxillary glands.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nerve Growth Factor,Ngfb,Beta-Ngf,Ngf
NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two a, one b and two g subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the b, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the b form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.