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NGF 2.5S antibody

Cat No. GTX10515

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, Dot, Double immunodiffusion, Neutralizing/Blocking
Reactivity Human, Mouse
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,000
ICC/IF Assay dependent
Dot 1:2,000
Double immunodiffusion Assay dependent
Neutralizing/Blocking Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Specificity/Sensitivity

May be used in monitoring for NGF (2.5S, β and 7S).
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

NGF-2.5S from male mouse submaxillary glands.

Purification

fractionated antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nerve growth factor , Ngfb , beta-NGF

Cellular Localization

Secreted

Background

NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two a, one b and two g subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the b, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the b form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.

Database

Research Area

REFERENCE
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SDS
PBS.pdf
Package List Price ($)
$ 319