GeneTex
United States (US)

NGF antibody [AS21]

Cat No. GTX16161

Host Mouse
Clonality Monoclonal
Clone Name AS21
Isotype IgG1
Application WB, ELISA, Neut
Reactivity Human, Mouse
APPLICATION

Application Note

Pairs with GTX16160 for EIA.
ELISA: Use at a concentration of 10 μg/ml for coating in a two site ELISA with biotin conjugated NGF antibody (GTX16160) for mouse NGF. Neut: This antibody has been used to neutralize > 90% human NGF bioactivity on PC12 cells at a 200 fold molar excess. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

27 kDa. ( Note )

Specificity/Sensitivity

Recognises 2.5S, 7S and bNGF.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

Protein G purified
Antibody Purity > 95 % by SDS PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nerve Growth Factor,Beta-Ngf,Hsan5,Ngfb,Ngf

Cellular Localization

Secreted

Background

Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture NGF induces the formation of neurite projections and in vivo may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease.

Database

Research Area

REFERENCE
Package List Price ($)
$ 319