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NHE-1 antibody

Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85046
Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85046
Anti-NHE-1 antibody used in Western Blot (WB). GTX85046

Cat No. GTX85046

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , MOLT4 Cell Lysate(GTX27912)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nhe-1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Nhe-1.The immunogen is located within amino acids 490 - 540 of Nhe-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725995

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

solute carrier family 9 member A1 , APNH , LIKNS , NHE-1 , NHE1 , PPP1R143

Cellular Localization

Membrane; Multi-pass membrane protein,Endoplasmic reticulum membrane,Cell membrane; Multi-pass membrane protein

Background

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

Database

Research Area

DATA IMAGES

Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85046

GTX85046 IHC-P Image

IHC-P analysis of human brain tissue using GTX85046 NHE-1 antibody.
Working concentration : 2.5 μg/ml

Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85046

GTX85046 IHC-P Image

IHC-P analysis of human brain tissue using GTX85046 NHE-1 antibody.
Working concentration : 20 μg/ml

Anti-NHE-1 antibody used in Western Blot (WB). GTX85046

GTX85046 WB Image

WB analysis of MOLT4 cell lysate using GTX85046 NHE-1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf