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NHE-1 antibody

Anti-NHE-1 antibody used in Western Blot (WB). GTX85047
Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85047
Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85047

Cat. No. GTX85047

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NHE-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Rat Kidney Tissue Lysate(GTX27944)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1.The immunogen is located within the last 50 amino acids of Nhe-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10724775

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 9 member A1 , APNH , LIKNS , NHE-1 , NHE1 , PPP1R143

Cellular Localization

Membrane; Multi-pass membrane protein,Endoplasmic reticulum membrane,Cell membrane; Multi-pass membrane protein

Background

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

Database

Research Area

DATA IMAGES

Anti-NHE-1 antibody used in Western Blot (WB). GTX85047

GTX85047 WB Image

WB analysis of rat kidney tissue lysate using GTX85047 NHE-1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85047

GTX85047 IHC-P Image

IHC-P analysis of human brain tissue using GTX85047 NHE-1 antibody.
Working concentration : 2.5 μg/ml

Anti-NHE-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85047

GTX85047 IHC-P Image

IHC-P analysis of human brain tissue using GTX85047 NHE-1 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Abdelazeem KNM et al. Cell Physiol Biochem 2017; 41 (6) : 2374-2382 Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells.
Application : WB
Reactivity : Human
Singh Y et al. J Biol Chem 2016; 291 (45) : 23662-23671 Alkaline Cytosolic pH and High Sodium Hydrogen Exchanger 1 (NHE1) Activity in Th9 Cells.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399