GeneTex
United States (US)

NHERF1 antibody [EBP-10]

Cat No. GTX29526

Host Mouse
Clonality Monoclonal
Clone Name EBP-10
Isotype IgG2b
Application WB, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

39 kDa. ( Note )

Specificity/Sensitivity

Human ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50 kDa (EBP50) / Na+/H+ exchange regulatory factor (NHERF)
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

>95% by SDS-PAGE
Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

NHERF1 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.

Database

Research Area

Package List Price ($)
$ 319