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NHERF1 antibody

Anti-NHERF1 antibody used in Western Blot (WB). GTX134431
Anti-NHERF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134431

Cat No. GTX134431

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

MCF-7

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.330 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NHERF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887279

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SLC9A3 regulator 1 , EBP50 , NHERF , NHERF-1 , NHERF1 , NPHLOP2

Cellular Localization

Cytoplasm,Apical cell membrane

Background

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-NHERF1 antibody used in Western Blot (WB). GTX134431

GTX134431 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NHERF1 antibody (GTX134431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 20802536 , and the observed expression level is based on the publication: PMID: 17078868

Anti-NHERF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134431

GTX134431 ICC/IF Image

NHERF1 antibody detects NHERF1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NHERF1 stained by NHERF1 antibody (GTX134431) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169