APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.330 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NHERF1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887279
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
SLC9A3 regulator 1 , EBP50 , NHERF , NHERF-1 , NHERF1 , NPHLOP2
Cellular Localization
Cytoplasm,Apical cell membrane
Background
This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
|
GTX134431 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NHERF1 antibody (GTX134431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 20802536 , and the observed expression level is based on the publication: PMID: 17078868
|
|
GTX134431 ICC/IF Image
NHERF1 antibody detects NHERF1 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NHERF1 stained by NHERF1 antibody (GTX134431) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
REFERENCE
There are currently no references for NHERF1 antibody (GTX134431). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NHERF1 antibody (GTX134431). Be the first to share your experience with this product.