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NHERF2 antibody, Internal

Anti-NHERF2 antibody, Internal used in Western Blot (WB). GTX89484

Cat. No. GTX89484

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all NHERF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89484-PEP).

Product Note

This antibody is expected to recognize isoforms b and e (NP_004776.3 and NP_001239005.1).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DLPGSDKDTEESGLH, from the internal region of the protein sequence according to NP_004776.3; NP_001239005.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SLC9A3 regulator 2 , E3KARP , NHE3RF2 , NHERF-2 , NHERF2 , OCTS2 , SIP-1 , SIP1 , TKA-1

Cellular Localization

Nucleus , Apical cell membrane

Background

This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-NHERF2 antibody, Internal used in Western Blot (WB). GTX89484

GTX89484 WB Image

WB analysis of Daudi lysate using GTX89484 NHERF2 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399