GeneTex
United States (US)

NHERF2 antibody [N2C3]

GTX101466_39742_WB_M_liver_19011506_444.jpg
GTX101466_39742_WB_19011506_832.jpg

Cat No. GTX101466

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

NT2D1 , mouse liver

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NHERF-2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Slc9A3 Regulator 2,E3Karp,Nhe3Rf2,Nherf-2,Nherf2,Octs2,Sip-1,Sip1,Tka-1,Slc9A3R2

Cellular Localization

Endomembrane system; Peripheral membrane protein , Nucleus

Background

NHE3 (SLC9A3; MIM 182307) is a sodium/hydrogen exchanger in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 (MIM 604990) and protein kinase A phosphorylation, may play a role in NHE3 regulation.[supplied by OMIM]

Database

DATA IMAGES
GTX101466_39742_WB_M_liver_19011506_444.jpg

GTX101466 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
15% SDS PAGE
GTX101466 diluted at 1:1000

GTX101466_39742_WB_19011506_832.jpg

GTX101466 WB Image

NHERF-2 antibody detects NHERF-2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with NHERF-2 antibody (GTX101466) diluted by 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169