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NHLRC1 antibody

Anti-NHLRC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32750
Anti-NHLRC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32750
Anti-NHLRC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32750
Anti-NHLRC1 antibody used in Western Blot (WB). GTX32750

Cat. No. GTX32750

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NHLRC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NHLRC1 (NP_940988.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NHL repeat containing E3 ubiquitin protein ligase 1 , EPM2A , EPM2B , MALIN , bA204B7.2

Cellular Localization

Endoplasmic reticulum,Nucleus

Background

The protein encoded by this gene is a single subunit E3 ubiquitin ligase. Laforin is polyubiquitinated by the encoded protein. Defects in this intronless gene lead to an accumulation of laforin and onset of Lafora disease, also known as progressive myoclonic epilepsy type 2 (EPM2).[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-NHLRC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32750

GTX32750 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX32750 NHLRC1 antibody.
Dilution : 1:100

Anti-NHLRC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32750

GTX32750 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32750 NHLRC1 antibody.
Dilution : 1:100

Anti-NHLRC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32750

GTX32750 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32750 NHLRC1 antibody.
Blue : DAPI

Anti-NHLRC1 antibody used in Western Blot (WB). GTX32750

GTX32750 WB Image

WB analysis of various sample lysates using GTX32750 NHLRC1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399