APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2 , PC-12 , Rat2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12
Predict Reactivity
Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human NHP2L1 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950997
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
small nuclear ribonucleoprotein 13 , 15.5K , FA-1 , FA1 , NHP2L1 , NHPX , OTK27 , SNRNP15-5 , SPAG12 , SSFA1
Cellular Localization
Nucleus , nucleolus
Background
Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247](/templates/default/js/lightbox/images/loading.gif "Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247")
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GTX109247 IHC-Wm Image
NHP2L1 antibody [N1C3] detects Nhp2l1 protein at nucleoli on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
NHP2L1 antibody [N1C3] (GTX109247) dilution: 1:200.
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GTX109247 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1(GTX109247) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109247 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1(GTX109247) antibody at 1:200 dilution.
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GTX109247 WB Image
NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (GTX109247) dilution: 1:1000
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GTX109247 WB Image
Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX109247 diluted at 1:1000
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GTX109247 WB Image
NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (GTX109247) dilution: 1:1000
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REFERENCE
There are currently no references for NHP2L1 antibody [N1C3] (GTX109247). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NHP2L1 antibody [N1C3] (GTX109247). Be the first to share your experience with this product.
![Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247](/upload/website/prouct_img/small/GTX109247/GTX109247_40023_IHC-Wm_Z_22111423_810.webp "Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109247](/upload/website/prouct_img/small/GTX109247/GTX109247_IHC_w_23060120_656.webp "Anti-NHP2L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109247](/upload/website/prouct_img/small/GTX109247/GTX109247_IFA_w_23060120_721.webp "Anti-NHP2L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247](/upload/website/prouct_img/small/GTX109247/GTX109247_40023_WB_R_w_23060120_538.webp "Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247](/upload/website/prouct_img/small/GTX109247/GTX109247_WB_w_23060120_423.webp "Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247](/upload/website/prouct_img/small/GTX109247/GTX109247_40023_WB_M_w_23060120_858.webp "Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247](/upload/website/prouct_img/normal/GTX109247/GTX109247_40023_IHC-Wm_Z_22111423_810.webp "Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109247](/upload/website/prouct_img/normal/GTX109247/GTX109247_IHC_w_23060120_656.webp "Anti-NHP2L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109247](/upload/website/prouct_img/normal/GTX109247/GTX109247_IFA_w_23060120_721.webp "Anti-NHP2L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247](/upload/website/prouct_img/normal/GTX109247/GTX109247_40023_WB_R_w_23060120_538.webp "Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247](/upload/website/prouct_img/normal/GTX109247/GTX109247_WB_w_23060120_423.webp "Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247")
![Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247](/upload/website/prouct_img/normal/GTX109247/GTX109247_40023_WB_M_w_23060120_858.webp "Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247")