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NIH 3T3 (mouse (mouse embryo) ) cell line slides

Cat. No. GTX25546

Application

ICC/IF

Species

Mouse
Package
5 slide ($199)
PRODUCT

Summary

NIH/3T3 (embryo)

Mouse NIH/3T3 cells were cultured in DMEM with 4 mM L-glutamine and harvested at the log phase of growth. The NIH/3T3 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 NIH/3T3 cells. A follow-up heat dehydration process was done to ensure the attachment of NIH/3T3 cells and the stability of cellular proteins.

APPLICATION

Application Note

NIH/3T3 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.

PROPERTIES

Storage

Stable for up to 6 months at 4˚C under desiccate conditions.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

REFERENCE

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REVIEW

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Package List Price ($)
$ 199