GeneTex
United States (US)

NIH-3T3 whole cell lysate

3T3-cell-lysate__GTX27901-1_18121409_288.jpg

Cat No. GTX27901

Application WB, Control
Species Mouse
PRODUCT

Product Description

NIH-3T3 embryonic cells grown to 85% confluency were harvested, homogenized in modified RIPA buffer (150 mM NaCl, 50 mM Tris-Cl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1 mM EDTA, 1 mM PMSF, 5 μg/ml Pepstatin A, 2 μg/ml Leupeptin, 1 mM sodium orthovanadate, 1 mM sodium fluoride.), and cleared by centrifugation. Total protein concentration of the lysate was determined using BCA protein assay (BSA was used as a standard). Lysate was mixed with Laemmli Sample Buffer containing reducing reagent and was boiled for 5 minutes. Lysate is supplied as 200 μg in 100 μl per vial (2.0 mg/ml total protein concentration) in 1X Laemmli sample buffer.
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Control Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

1 x SDS sample buffer containing 5% β-mercaptoethanol

Concentration

1mg/ml

Purification

Whole Cell Lysate

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
3T3-cell-lysate__GTX27901-1_18121409_288.jpg

GTX27901 Control Image

SDS-PAGE image of 3T3 Whole Cell Lysate (GTX27901) loaded at (A) 10 ug and (B) 20 ug protein/lane. Proteins were separated on 8-16% Tris-HCl gradient gel.

Package List Price ($)
$ 149