GeneTex

  • Country / Location Selection

United States (US)

NIP7 antibody

Anti-NIP7 antibody used in Western Blot (WB). GTX120926
Anti-NIP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120926

Cat. No. GTX120926

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NIP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NIP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885552

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolar pre-rRNA processing protein NIP7 , CGI-37 , HSPC031 , KD93

Cellular Localization

Nucleus , nucleolus

Background

Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.

Database

DATA IMAGES

Anti-NIP7 antibody used in Western Blot (WB). GTX120926

GTX120926 WB Image

NIP7 antibody detects NIP7 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
NIP7 antibody (GTX120926) dilution: 1:1000

Anti-NIP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120926

GTX120926 ICC/IF Image

NIP7 antibody detects NIP7 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NIP7 protein stained by NIP7 antibody (GTX120926) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

There are currently no references for NIP7 antibody (GTX120926). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for NIP7 antibody (GTX120926). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169