GeneTex
United States (US)

NIPBL antibody, Internal

GTX88110_1057_IHC-P_18122102_870.jpg
GTX88110_133_ICC-IF_18122102_845.jpg

Cat No. GTX88110

Host Goat
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 10μg/ml
IHC-P 2μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm and nucleus of HeLa cells.

Not tested in other applications.

Calculated MW

316 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88110-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_597677.2; NP_056199.2).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RPDSPRVKQGDSNK, from the internal region of the protein sequence according to NP_597677.2; NP_056199.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nipbl, Cohesin Loading Factor,Cdls,Cdls1,Idn3,Idn3-B,Scc2,Nipbl

Background

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88110_1057_IHC-P_18122102_870.jpg

GTX88110 IHC-P Image

IHC-P analysis of human kidney using GTX88110 NIPBL antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2µg/ml

GTX88110_133_ICC-IF_18122102_845.jpg

GTX88110 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX88110 NIPBL antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

Package List Price ($)
$ 329