APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 2.5-3.8μg/ml |
|
Note :
IHC-P
Human Thymus shows speckled staining of nuclei.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX25300-PEP).
Product Note
This antibody is expected to recognise all three human isoforms of this protein.
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-EAWPGKKPTPSLLI, from the C Terminus of the protein sequence according to NP_002704.1; NP_054829.2; NP_612568.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein phosphatase 1 regulatory subunit 8 , ARD-1 , ARD1 , NIPP-1 , NIPP1 , PRO2047
Cellular Localization
Nucleus , Nucleus speckle
Background
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
|
GTX25300 WB Image
WB analysis of HeLa (A), HepG2 (B), Jurkat (C) and NIH-3T3 (D) nuclear lysates using GTX25300 NIPP1 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX25300 IHC-P Image
IHC-P analysis of human thymus using GTX25300 NIPP1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for NIPP1 antibody, C-term (GTX25300). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NIPP1 antibody, C-term (GTX25300). Be the first to share your experience with this product.
