GeneTex
United States (US)

NIR1 antibody [C3], C-term

GTX108085_39728_20171215_WB_19011507_123.jpg
GTX108085_39728_IFA_19011507_526.jpg

Cat No. GTX108085

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NIR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pitpnm Family Member 3,Ackr6,Cord5,Nir1,Rdgba3,Pitpnm3

Cellular Localization

Endomembrane system; Peripheral membrane protein

Background

PITPNM3 belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein (Ocaka et al., 2005 [PubMed 15627748]).[supplied by OMIM]

Database

DATA IMAGES
GTX108085_39728_20171215_WB_19011507_123.jpg

GTX108085 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NIR1 antibody [C3], C-term (GTX108085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108085_39728_IFA_19011507_526.jpg

GTX108085 ICC/IF Image

NIR1 antibody [C3], C-term detects PITPNM3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min.
Green: PITPNM3 protein stained by NIR1 antibody [C3], C-term (GTX108085) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169