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NIT1 antibody [3A11]

Overexpression
Anti-NIT1 antibody [3A11] used in Western Blot (WB). GTX84041
Anti-NIT1 antibody [3A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84041
Anti-NIT1 antibody [3A11] used in Flow cytometry (FACS). GTX84041
Anti-NIT1 antibody [3A11] used in IHC (Paraffin sections) (IHC-P). GTX84041
Anti-NIT1 antibody [3A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84041
Anti-NIT1 antibody [3A11] used in Western Blot (WB). GTX84041
Anti-NIT1 antibody [3A11] used in IHC (Paraffin sections) (IHC-P). GTX84041

Cat. No. GTX84041

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all NIT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitrilase 1

Background

Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells | this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.

Database

Research Area

DATA IMAGES

Anti-NIT1 antibody [3A11] used in Western Blot (WB). GTX84041

GTX84041 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84041 NIT1 antibody [3A11].
Loading : 10 ug per lane
Dilution : 1:500

Anti-NIT1 antibody [3A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84041

GTX84041 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84041 NIT1 antibody [3A11].

Anti-NIT1 antibody [3A11] used in Flow cytometry (FACS). GTX84041

GTX84041 FACS Image

FACS analysis of HEK293T cells transfected with either NIT1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84041 NIT1 antibody [3A11].

Anti-NIT1 antibody [3A11] used in IHC (Paraffin sections) (IHC-P). GTX84041

GTX84041 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84041 NIT1 antibody [3A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NIT1 antibody [3A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84041

GTX84041 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NIT1 plasmid using GTX84041 NIT1 antibody [3A11].

Anti-NIT1 antibody [3A11] used in Western Blot (WB). GTX84041

GTX84041 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NIT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84041 NIT1 antibody [3A11].
Loading : 5 ug per lane

Anti-NIT1 antibody [3A11] used in IHC (Paraffin sections) (IHC-P). GTX84041

GTX84041 IHC-P Image

IHC-P analysis of kidney tissue using GTX84041 NIT1 antibody [3A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519