NK1.1 antibody [PK136] (APC)

Cat. No. GTX01478-07

Host	Mouse
Clonality	Monoclonal
Clone Name	PK136
Isotype	IgG2a
Application	FACS
Reactivity	Mouse
Package	100 μg ($269)

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View product citations for antibody GTX01478-07 on CiteAb

PRODUCT

Summary

The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	Assay dependent

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Membrane

Database

Gene ID: 17059 Klrb1c
UniProt: P27814 Klrb1c

Research Area

DATA IMAGES

GTX01478-07 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01478-07 NK1.1 antibody [PK136] (APC).
Right panel : co-stained with NK1.1 antibody [PK136] (APC) and Mouse CD3e antibody (FITC)
Left panel : co-stained with isotype control and Mouse CD3e antibody (FITC)
antibody amount : 0.125 μg (5 μl)