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NKCC2 antibody, N-term

Anti-NKCC2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47166
Anti-NKCC2 antibody, N-term used in Western Blot (WB). GTX47166
Anti-NKCC2 antibody, N-term used in Western Blot (WB). GTX47166

Cat. No. GTX47166

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
50 μg ($409)
See all NKCC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

A549 , Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human NKCC2.

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_11167952

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 12 member 1 , BSC1 , NKCC2

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-NKCC2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47166

GTX47166 IHC-P Image

IHC-P analysis of human kidney tissue using GTX47166 SLC12A1 antibody at 4.0-8.0μg/ml.

Anti-NKCC2 antibody, N-term used in Western Blot (WB). GTX47166

GTX47166 WB Image

WB analysis of A549 cells using GTX47166 SLC12A1 antibody at 1μg/ml.

Anti-NKCC2 antibody, N-term used in Western Blot (WB). GTX47166

GTX47166 WB Image

WB analysis of Jurkat cells using GTX47166 SLC12A1 antibody at 0.2-1μg/ml.
Lane A : marker
Lane B : Jurkat cells

REFERENCE

Application Reference

Li Q et al. Theranostics 2021; 11 (20) : 10064-10073 Heterogeneity of cell composition and origin identified by single-cell transcriptomics in renal cysts of patients with autosomal dominant polycystic kidney disease.
Application : IHC-P
Cooper DL et al. PLoS One 2014; 9 (2) : e89087 exacerbation of *-induced renal injury by concomitant administration of * in rats.
Application : IHC-P
Reactivity : Rat
Lan YF et al. J Am Soc Nephrol 2012; 23 (12) : 2012-23 MicroRNA-494 reduces ATF3 expression and promotes AKI.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409