GeneTex
United States (US)

NKG2A antibody [N1N2], N-term

GTX108498_WB_19011507_470.jpg
GTX108498_39862_IFA_19011507_700.jpg
GTX108498_39862_IHC_19011507_504.jpg

Cat No. GTX108498

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NKG2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Killer Cell Lectin Like Receptor C1,Cd159A,Nkg2,Nkg2A,Klrc1

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. [provided by RefSeq]

Database

DATA IMAGES
GTX108498_WB_19011507_470.jpg

GTX108498 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hep G2 (GTX27900)
12% SDS PAGE
NKG2-A (CD159a) antibody
GTX108498 diluted at 1:1000

GTX108498_39862_IFA_19011507_700.jpg

GTX108498 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using KLRC1(GTX108498) antibody at 1:200 dilution.

GTX108498_39862_IHC_19011507_504.jpg

GTX108498 IHC-P Image

KLRC1 antibody [N1N2], N-term detects KLRC1 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
KLRC1 antibody [N1N2], N-term (GTX108498) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169