GeneTex
United States (US)

NKG2D antibody [N3C2], Internal

GTX104761_39666_IHC_19011506_360.jpg
GTX104761_40205_20141229_WB_spleen_19011506_263.jpg

Cat No. GTX104761

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

human spleen

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human NKG2D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD314 antibody, D12S2489E antibody, FLJ17759 antibody, FLJ75772 antibody, KLR antibody, NKG2-D antibody, NKG2D antibody, KLRK1 antibody, NK cell receptor D antibody, NKG2-D-activating NK receptor antibody, NKG2-D type II integral membrane protein antibody, killer cell lectin-like receptor subfamily K, member 1 antibody

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. [provided by RefSeq]

Database

DATA IMAGES
GTX104761_39666_IHC_19011506_360.jpg

GTX104761 IHC-P Image

NKG2D antibody [N3C2], Internal detects NKG2D protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
NKG2D antibody [N3C2], Internal (GTX104761) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104761_40205_20141229_WB_spleen_19011506_263.jpg

GTX104761 WB Image

NKG2D antibody detects NKG2D protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with NKG2D antibody (GTX104761) at a dilution of 1:1000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169