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NKG2D antibody [N3C2], Internal

Anti-NKG2D antibody [N3C2], Internal used in Western Blot (WB). GTX104761
Anti-NKG2D antibody [N3C2], Internal used in Western Blot (WB). GTX104761
Anti-NKG2D antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104761
Anti-NKG2D antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104761

Cat. No. GTX104761

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

human spleen

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human NKG2D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241111

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

killer cell lectin like receptor K1 , CD314 , D12S2489E , KLR , NKG2-D , NKG2D

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NKG2D antibody [N3C2], Internal used in Western Blot (WB). GTX104761

GTX104761 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NKG2D antibody [N3C2], Internal used in Western Blot (WB). GTX104761

GTX104761 WB Image

NKG2D antibody detects NKG2D protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with NKG2D antibody (GTX104761) at a dilution of 1:1000.

Anti-NKG2D antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104761

GTX104761 IHC-P Image

NKG2D antibody [N3C2], Internal detects NKG2D protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
NKG2D antibody [N3C2], Internal (GTX104761) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NKG2D antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104761

GTX104761 ICC/IF Image

NKG2D antibody [N3C2], Internal detects NKG2D protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NKG2D stained by NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169