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NKX2.1 antibody [8G7G3/1]

Anti-NKX2.1 antibody [8G7G3/1] used in IHC (Paraffin sections) (IHC-P). GTX34907
Anti-NKX2.1 antibody [8G7G3/1] used in IHC (Paraffin sections) (IHC-P). GTX34907

Cat. No. GTX34907

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8G7G3/1

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($529)
See all NKX2.1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat full length TTF-1 recombinant protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor; may play a role in organogenesis and/or reproductive maturation and development [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-NKX2.1 antibody [8G7G3/1] used in IHC (Paraffin sections) (IHC-P). GTX34907

GTX34907 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX34907 NKX2.1 antibody [8G7G3/1].

Anti-NKX2.1 antibody [8G7G3/1] used in IHC (Paraffin sections) (IHC-P). GTX34907

GTX34907 IHC-P Image

IHC-P analysis of human lung adenocarcinoma tissue using GTX34907 NKX2.1 antibody [8G7G3/1].

REFERENCE

Application Reference

F Ciarpella et al. STAR Protoc 2023; 4 (3) : 102413 Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells
Reactivity : Mouse
Ciarpella F et al. iScience 2021; 24 (12) : 103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
Application : IHC-P
Reactivity : Mouse

Clone Reference

Yu S et al. Dev Biol 2010; 347 (1):92-108 Fetal and postnatal lung defects reveal a novel and required role for Fgf8 in lung development.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529