APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.25 - 0.5 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lung Tissue Lysate(GTX27917) , Human Lung Tissue Slide
Product Note
NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1.The immunogen is located within the first 50 amino acids of NKX2-1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NK2 homeobox 1 , BCH , BHC , NK-2 , NKX2.1 , NKX2A , NMTC1 , T/EBP , TEBP , TITF1 , TTF-1 , TTF1
Cellular Localization
Nucleus
Background
This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TFF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Apr 2011]
Database
Research Area
DATA IMAGES
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GTX31847 IHC-P Image
IHC-P analysis of human lung tissue using GTX31847 NKX2.1 antibody.
Working concentration : 20 μg/ml
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GTX31847 WB Image
WB analysis of human lung tissue lysate using GTX31847 NKX2.1 antibody.
Working concentration : (A) 0.25 and (B) 0.5 μg/ml
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GTX31847 IHC-P Image
IHC-P analysis of human lung tissue using GTX31847 NKX2.1 antibody.
Working concentration : 2.5 μg/ml
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REFERENCE
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REVIEW
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