GeneTex
United States (US)

NKX2.1 antibody

Anti-NKX2.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31847
Anti-NKX2.1 antibody used in Western Blot (WB). GTX31847
Anti-NKX2.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31847

Cat No. GTX31847

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25 - 0.5 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Human Lung Tissue Lysate(GTX27917) , Human Lung Tissue Slide

Specificity/Sensitivity

NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1.The immunogen is located within the first 50 amino acids of NKX2-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nk2 Homeobox 1,Bch,Bhc,Nk-2,Nkx2.1,Nkx2A,Nmtc1,T/Ebp,Tebp,Titf1,Ttf-1,Ttf1,Nkx2-1

Background

This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TFF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES
Anti-NKX2.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31847

GTX31847 IHC-P Image

IHC-P analysis of human lung tissue using GTX31847 NKX2.1 antibody.
Working concentration : 20 μg/ml

Anti-NKX2.1 antibody used in Western Blot (WB). GTX31847

GTX31847 WB Image

WB analysis of human lung tissue lysate using GTX31847 NKX2.1 antibody.
Working concentration : (A) 0.25 and (B) 0.5 μg/ml

Anti-NKX2.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31847

GTX31847 IHC-P Image

IHC-P analysis of human lung tissue using GTX31847 NKX2.1 antibody.
Working concentration : 2.5 μg/ml

Package List Price ($)
$ 329