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NLK antibody [N3C3]

Overexpression
Anti-NLK antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107910
Anti-NLK antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107910
Anti-NLK antibody [N3C3] used in Western Blot (WB). GTX107910
Anti-NLK antibody [N3C3] used in Western Blot (WB). GTX107910
Anti-NLK antibody [N3C3] used in Western Blot (WB). GTX107910

Cat. No. GTX107910

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NLK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Mouse brain , HeLa , NLK-transfected 293T

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NLK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951000

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nemo like kinase

Cellular Localization

Nucleus , Cytoplasm

Background

Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.

Database

Research Area

DATA IMAGES

Anti-NLK antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107910

GTX107910 IHC-P Image

NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (GTX107910) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NLK antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107910

GTX107910 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(GTX107910) antibody at 1:100 dilution.

Anti-NLK antibody [N3C3] used in Western Blot (WB). GTX107910

GTX107910 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX107910 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NLK antibody [N3C3] used in Western Blot (WB). GTX107910

GTX107910 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
NLK antibody
GTX107910 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NLK antibody [N3C3] used in Western Blot (WB). GTX107910

GTX107910 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NLK antibody [N3C3] (GTX107910) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169