APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 10μg/ml |
| Assay dependent |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of U937 cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89829-PEP).
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-EKPELTVVFEPSW, from the C Terminus of the protein sequence according to NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NLR family pyrin domain containing 3 , AGTAVPRL , AII , AVP , C1orf7 , CIAS1 , CLR1.1 , DFNA34 , FCAS , FCAS1 , FCU , KEFH , MWS , NALP3 , PYPAF1
Cellular Localization
Cytoplasm, cytosol , Endoplasmic reticulum , Secreted , Nucleus
Background
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
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GTX89829 IHC-P Image
IHC-P analysis of human colon tissue using GTX89829 Cryopyrin ( C Terminus) antibody, C-term.
Antigen retrieval : Citrate buffer pH 6.0
Dilution : 4μg/ml
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GTX89829 IHC-P Image
IHC-P analysis of human colon tissue without using GTX89829 Cryopyrin ( C Terminus) antibody, C-term.(Negative control)
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GTX89829 FACS Image
FACS analysis of PFA-fixed U937 cells using GTX89829 Cryopyrin ( C Terminus) antibody, C-term.
Blue line : Primary antibody
Black line : Negative control: Unimmunized goat IgG
Permeabilization : 0.5% Triton
Dilution : 10ug/ml
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GTX89829 ICC/IF Image
ICC/IF analysis of PFA-fixed U937 cells using GTX89829 Cryopyrin ( C Terminus) antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG (10ug/ml)
Permeabilization : 0.15% Triton
Dilution : 10ug/ml
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REFERENCE
There are currently no references for NLRP3 ( C Terminus) antibody, C-term (GTX89829). Be the first to share your publications with this product.
REVIEW
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