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NLRP3 antibody [C3], C-term

KO/KD Orthogonal
Anti-NLRP3 antibody [C3], C-term used in Western Blot (WB). GTX106313
Anti-NLRP3 antibody [C3], C-term used in Western Blot (WB). GTX106313

Cat. No. GTX106313

Reference 1
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all NLRP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NLRP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NLR family pyrin domain containing 3 , AGTAVPRL , AII , AVP , C1orf7 , CIAS1 , CLR1.1 , DFNA34 , FCAS , FCAS1 , FCU , KEFH , MWS , NALP3 , PYPAF1

Cellular Localization

Cytoplasm, cytosol,Endoplasmic reticulum,Secreted,Nucleus

Background

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NLRP3 antibody [C3], C-term used in Western Blot (WB). GTX106313

GTX106313 WB Image

Orthogonal

Boiled and unboiled THP-1 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NLRP3 antibody [C3], C-term (GTX106313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-NLRP3 antibody [C3], C-term used in Western Blot (WB). GTX106313

GTX106313 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NLRP3 antibody [C3], C-term (GTX106313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hao Li et al. Antioxidants (Basel) 2021; 10 (8) Isoorientin Attenuated the Pyroptotic Hepatocyte Damage Induced by Benzo[a]pyrene via ROS/NF-κB/NLRP3/Caspase-1 Signaling Pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

NLRP3 antibody [C3], C-term Cat. No. GTX106313

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-NLRP3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX106313
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : THP-1 cells
Lane Description :
SM=4% starting material
UB=4% unbound fraction
Figure Note : Antibody-beads conjugates were prepared by adding 2 μg to 500 μl of IP Lysis Buffer in a microcentrifuge tube, together with with 30 μl of Dynabeads protein A- (for rabbit antibodies) or protein G- (for mouse and rat antibodies) beads. Tubes were rocked for ~1 hr at 4ºC followed by two washes to remove unbound antibodies.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-NLRP3 antibody [C3], C-term used in Western Blot (WB). GTX106313
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 WT and NLRP3 KO cells treated or not with PMA
Amount used : 20μg
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-NLRP3 antibody [C3], C-term used in Western Blot (WB). GTX106313
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 WT and NLRP3 KO cells cultured medium
Amount used : 20μg
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
Package List Price ($)
$ 419
$ 169