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NLRP3 antibody

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763
Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763

Cat No. GTX00763

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Rat
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells

Note :

IHC-P
Recommed a heat mediated antigen retrieval

Not tested in other applications.

Calculated MW

118 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was raised against a synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NLR family pyrin domain containing 3 , AGTAVPRL , AII , AVP , C1orf7 , CIAS1 , CLR1.1 , DFNA34 , FCAS , FCAS1 , FCU , KEFH , MWS , NALP3 , PYPAF1

Cellular Localization

Cytoplasm, cytosol,Endoplasmic reticulum,Secreted,Nucleus

Background

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763

GTX00763 FACS Image

FACS analysis of THP-1 cells using GTX00763 NLRP3 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Blank control
Antibody amount : 1μg/1x10⁶ cells

Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763

GTX00763 FACS Image

FACS analysis of U937 cells using GTX00763 NLRP3 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Blank control
Antibody amount : 1μg/1x10⁶ cells

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00763 NLRP3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat colon tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat colon tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763

GTX00763 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX00763 NLRP3 antibody.
Antigen retrieval : Enzyme antigen retrieval was performed for 15 mins
Dilution : 2μg/ml

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX00763 NLRP3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins.
Dilution : 1μg/ml

Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763

GTX00763 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX00763 NLRP3 antibody.
Antigen retrieval : Enzyme antigen retrieval was performed for 15 mins
Dilution : 2μg/ml

REFERENCE
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REVIEW
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Package List Price ($)
$ 329