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NLRP3 antibody

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in Western Blot (WB). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763
Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

Cat. No. GTX00763

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all NLRP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells

Note :

IHC-P
Recommed a heat mediated antigen retrieval
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was raised against a synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NLR family pyrin domain containing 3 , AGTAVPRL , AII , AVP , C1orf7 , CIAS1 , CLR1.1 , DFNA34 , FCAS , FCAS1 , FCU , KEFH , MWS , NALP3 , PYPAF1

Cellular Localization

Cytoplasm, cytosol,Endoplasmic reticulum,Secreted,Nucleus

Background

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00763 NLRP3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763

GTX00763 FACS Image

FACS analysis of U937 cells using GTX00763 NLRP3 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Blank control
Antibody amount : 1μg/1x10⁶ cells

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763

GTX00763 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX00763 NLRP3 antibody.
Antigen retrieval : Enzyme antigen retrieval was performed for 15 mins
Dilution : 2μg/ml

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat colon tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in Western Blot (WB). GTX00763

GTX00763 WB Image

WB analysis of various samples using GTX00763 NLRP3 antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : U87 whole cell lysate
Lane 4 : CEM whole cell lysate
Dilution : 0.5 μg/mL
Loading : 50μg

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX00763 NLRP3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins.
Dilution : 1μg/ml

Anti-NLRP3 antibody used in Flow cytometry (FACS). GTX00763

GTX00763 FACS Image

FACS analysis of THP-1 cells using GTX00763 NLRP3 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Blank control
Antibody amount : 1μg/1x10⁶ cells

Anti-NLRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00763

GTX00763 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX00763 NLRP3 antibody.
Antigen retrieval : Enzyme antigen retrieval was performed for 15 mins
Dilution : 2μg/ml

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00763

GTX00763 IHC-P Image

IHC-P analysis of rat colon tissue section using GTX00763 NLRP3 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins
Dilution : 1μg/ml

REFERENCE

Application Reference

Lou Q et al. Int J Nanomedicine 2023; 18 : 2447-2463 Suppression of NLRP3/Caspase-1/GSDMD Mediated Corneal Epithelium Pyroptosis Using Melatonin-Loaded Liposomes to Inhibit Benzalkonium Chloride-Induced Dry Eye Disease.
Arab HH et al. Pharmaceuticals (Basel) 2022; 15 (7) Repositioning Linagliptin for the Mitigation of Cadmium-Induced Testicular Dysfunction in Rats: Targeting HMGB1/TLR4/NLRP3 Axis and Autophagy.
Application : IHC-P
Reactivity : Rat
Lo J et al. J Inflamm Res 2022; 15 : 5347-5359 Punicalagin Attenuates LPS-Induced Inflammation and ROS Production in Microglia by Inhibiting the MAPK/NF-κB Signaling Pathway and NLRP3 Inflammasome Activation.
Arab HH et al. Pharmaceuticals (Basel) 2022; 15 (11) Topiramate Reprofiling for the Attenuation of Cadmium-Induced Testicular Impairment in Rats: Role of NLRP3 Inflammasome and AMPK/mTOR-Linked Autophagy.
Erin Clough et al. J Neuroimmune Pharmacol 2021; 16 (4) : 770-784 Mitochondrial Dynamics in SARS-COV2 Spike Protein Treated Human Microglia: Implications for Neuro-COVID.
Application : ICC/IF
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 429