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NLRP3 antibody

Orthogonal
Anti-NLRP3 antibody used in Western Blot (WB). GTX133569
Anti-NLRP3 antibody used in Western Blot (WB). GTX133569
Anti-NLRP3 antibody used in Western Blot (WB). GTX133569
Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX133569

Cat. No. GTX133569

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NLRP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NLRP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887025

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NLR family pyrin domain containing 3 , AGTAVPRL , AII , AVP , C1orf7 , CIAS1 , CLR1.1 , DFNA34 , FCAS , FCAS1 , FCU , KEFH , MWS , NALP3 , PYPAF1

Cellular Localization

Cytoplasm

Background

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NLRP3 antibody used in Western Blot (WB). GTX133569

GTX133569 WB Image

Orthogonal

Boiled and unboiled THP-1 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NLRP3 antibody (GTX133569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-NLRP3 antibody used in Western Blot (WB). GTX133569

GTX133569 WB Image

Untreated (–) and treated (+) THP-1 whole cell extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NLRP3 antibody (GTX133569) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NLRP3 antibody used in Western Blot (WB). GTX133569

GTX133569 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NLRP3 antibody (GTX133569) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NLRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX133569

GTX133569 IHC-P Image

NLRP3 antibody detects NLRP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
NLRP3 stained by NLRP3 antibody (GTX133569) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Guo Z et al. Bioengineered 2021; 12 (2) : 11030-11040 Zafirlukast ameliorates Docetaxel-induced activation of NOD-like receptor protein 3 (NLRP3) inflammasome, mediated by sirtuin1 (SIRT1) in hepatocytes.
Application : WB
Reactivity : Human
Garc?a-Baos A. et al. bioRxiv 2021; (Epub) Cannabidiol attenuates cognitive deficits and neuroinflammation induced by early alcohol exposure in a mice model.
Application : WB
Reactivity : Mouse
Wu CY et al. J Cell Mol Med 2020; (Epub) Tris DBA ameliorates IgA nephropathy by blunting the activating signal of NLRP3 inflammasome through SIRT1- and SIRT3-mediated autophagy induction.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169