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NMD3 antibody

Anti-NMD3 antibody used in Western Blot (WB). GTX130480
Anti-NMD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130480

Cat. No. GTX130480

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NMD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Human, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NMD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886284

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NMD3 ribosome export adaptor , CGI-07

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm , nucleolus

Background

Ribosomes are composed of 60S and 40S subunits that are assembled in the nucleolus and exported to the cytoplasm through nuclear pore complexes in the nuclear envelope. NMD3 is an adaptor for 60S subunit export via the CRM1 (XPO1; MIM 602559) pathway (Trotta et al., 2003 [PubMed 12773398]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NMD3 antibody used in Western Blot (WB). GTX130480

GTX130480 WB Image

NMD3 antibody detects NMD3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with NMD3 antibody (GTX130480) at a dilution of 1:500.

Anti-NMD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130480

GTX130480 IHC-P Image

NMD3 antibody detects NMD3 protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
NMD3 antibody (GTX130480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169