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NMDAR1 antibody, Internal

Anti-NMDAR1 antibody, Internal used in Western Blot (WB). GTX89302

Cat No. GTX89302

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89302-PEP).

Specificity/Sensitivity

This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TQERVNNSNKKE, from the internal region of the protein sequence according to NP_000823.4; NP_067544.1; NP_015566.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Glutamate Ionotropic Receptor Nmda Type Subunit 1 , Glun1 , Mrd8 , Ndhmsd , Ndhmsr , Nmd-R1 , Nmda1 , Nmdar1 , Nr1 , Grin1

Cellular Localization

Enriched in post-synaptic plasma membrane and post-synaptic densities.

Background

The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NMDAR1 antibody, Internal used in Western Blot (WB). GTX89302

GTX89302 WB Image

WB analysis of human amylgada (A) and rat (B) brain lysate using GTX89302 NMDAR1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf