GeneTex
United States (US)

NMDAR1 antibody

GTX133097_43404_20181109_WB_M_R_19021719_489.jpg
GTX133097_42508_20160720_IHC-P_R_18121410_829.jpg
GTX133097_42508_20170220_IHC-Fr_M_18121410_924.jpg
GTX133097_42508_20170503_IFA_R_18121410_438.jpg

Cat No. GTX133097

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

mouse brain , mouse fetal brain , rat brain

Predict Reactivity

Human, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NMDAR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GluN1 antibody , GluRdelta1 antibody , GluRzeta1 antibody , Grin1 antibody , M100174 antibody , NMDAR1 antibody , NMDAR1 antibody , NR1 antibody , Nmdar antibody , Rgsc174 antibody , glutamate receptor, ionotropic, NMDA1 (zeta 1) antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein (By similarity) , Cell junction , synapse , postsynaptic cell membrane (By similarity) , postsynaptic cell membrane , postsynaptic density (By similarity)

Background

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Modulated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

Database

Research Area

DATA IMAGES
GTX133097_43404_20181109_WB_M_R_19021719_489.jpg

GTX133097 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NMDAR1 antibody (GTX133097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133097_42508_20160720_IHC-P_R_18121410_829.jpg

GTX133097 IHC-P Image

NMDAR1 antibody detects NMDAR1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NMDAR1 antibody (GTX133097) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133097_42508_20170220_IHC-Fr_M_18121410_924.jpg

GTX133097 IHC-Fr Image

NMDAR1 antibody detects NMDAR1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133097_42508_20170503_IFA_R_18121410_438.jpg

GTX133097 ICC/IF Image

NMDAR1 antibody detects NMDAR1 protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue:Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169