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NMDAR1 antibody

Anti-NMDAR1 antibody used in Western Blot (WB). GTX133097
Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097
Anti-NMDAR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133097
Anti-NMDAR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133097
Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097

Cat. No. GTX133097

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all NMDAR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Predict Reactivity

Human, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of mouse NMDAR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886824

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate receptor, ionotropic, NMDA1 (zeta 1) , GluN1 , GluRdelta1 , GluRzeta1 , M100174 , NMD-R1 , NMDAR1 , NR1 , Nmdar , Rgsc174

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane , postsynaptic cell membrane , postsynaptic density

Background

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Modulated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

Database

Research Area

DATA IMAGES

Anti-NMDAR1 antibody used in Western Blot (WB). GTX133097

GTX133097 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NMDAR1 antibody (GTX133097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097

GTX133097 ICC/IF Image

NMDAR1 antibody detects NMDAR1 protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue:Fluoroshield with DAPI (GTX30920).
Anti-NMDAR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133097

GTX133097 IHC-P Image

NMDAR1 antibody detects NMDAR1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NMDAR1 antibody (GTX133097) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMDAR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133097

GTX133097 IHC-Fr Image

NMDAR1 antibody detects NMDAR1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097

GTX133097 ICC/IF Image

NMDAR1 antibody detects NMDAR1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR1 stained by NMDAR1 antibody (GTX133097) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Wiatrak B et al. Mol Biol Rep 2022; 49 (11) : 10443-10455 Impact of NMDA receptor activation on DNA damage in PC12 neuron-like cell cultures in the presence of β-amyloid peptides.
Ko CY et al. Front Aging Neurosci 2021; 13 : 774477 Alleviative Effect of Alpha-Lipoic Acid on Cognitive Impairment in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Rats.
Application : WB
Reactivity : Rat
Lo Cascio F et al. J Biol Chem 2020; 295 (44) : 14807-14825 Modulating disease-relevant tau oligomeric strains by small molecules.
Application : WB
Reactivity : Mouse
Algamal M et al. J Neurotrauma 2019; (Epub) Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress.
Application : WB
Reactivity : Mouse
Meng G et al. EBioMedicine 2019; Gut microbe-derived metabolite trimethylamine N-oxide activates the cardiac autonomic nervous system and facilitates ischemia-induced ventricular arrhythmia via two different pathways.
Application : IHC-P
Reactivity : Dog
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169