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NMDAR1 antibody

Anti-NMDAR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133097
Anti-NMDAR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133097
Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097
Anti-NMDAR1 antibody used in Western Blot (WB). GTX133097

Cat No. GTX133097

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat, Dog
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

mouse brain , mouse fetal brain , rat brain

Predict Reactivity

Human(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NMDAR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate receptor, ionotropic, NMDA1 (zeta 1) , GluN1 , GluRdelta1 , GluRzeta1 , M100174 , NMD-R1 , NMDAR1 , NR1 , Nmdar , Rgsc174

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane , postsynaptic cell membrane , postsynaptic density

Background

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Modulated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

Database

Research Area

DATA IMAGES
Anti-NMDAR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133097

GTX133097 IHC-P Image

NMDAR1 antibody detects NMDAR1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NMDAR1 antibody (GTX133097) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMDAR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133097

GTX133097 IHC-Fr Image

NMDAR1 antibody detects NMDAR1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097

GTX133097 ICC/IF Image

NMDAR1 antibody detects NMDAR1 protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue:Fluoroshield with DAPI (GTX30920).

Anti-NMDAR1 antibody used in Western Blot (WB). GTX133097

GTX133097 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NMDAR1 antibody (GTX133097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169