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NMDAR2A antibody [C1C2], Internal

Anti-NMDAR2A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113717
Anti-NMDAR2A antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113717

Cat. No. GTX113717

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all NMDAR2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

Predict Reactivity

Mouse, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human NMDAR2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate ionotropic receptor NMDA type subunit 2A , EPND , FESD , GluN2A , LKS , NMDAR2A , NR2A

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein

Background

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NMDAR2A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113717

GTX113717 IHC-P Image

NMDAR2A antibody [C1C2], Internal detects GRIN2A protein at cytosol and nucleus on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
NMDAR2A antibody [C1C2], Internal (GTX113717) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NMDAR2A antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113717

GTX113717 ICC/IF Image

NMDAR2A antibody [C1C2], Internal detects NMDAR2A protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR2A protein stained by NMDAR2A antibody [C1C2], Internal (GTX113717) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Niedzi??ka SM et al. Commun Biol 2024; 7 (1) : 213 The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169