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NMDAR2A antibody

Anti-NMDAR2A antibody used in Western Blot (WB). GTX103558
Anti-NMDAR2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103558
Anti-NMDAR2A antibody used in Western Blot (WB). GTX103558
Anti-NMDAR2A antibody used in Western Blot (WB). GTX103558
Anti-NMDAR2A antibody used in IHC (Paraffin sections) (IHC-P). GTX103558
Anti-NMDAR2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103558

Cat. No. GTX103558

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human NMDAR2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727703

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate ionotropic receptor NMDA type subunit 2A , EPND , FESD , GluN2A , LKS , NMDAR2A , NR2A

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein

Background

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NMDAR2A antibody used in Western Blot (WB). GTX103558

GTX103558 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
5% SDS PAGE
GTX103558 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NMDAR2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103558

GTX103558 ICC/IF Image

NMDAR2A antibody detects NMDAR2A protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR2A protein stained by NMDAR2A antibody (GTX103558) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NMDAR2A antibody used in Western Blot (WB). GTX103558

GTX103558 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (GTX103558) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NMDAR2A antibody used in Western Blot (WB). GTX103558

GTX103558 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (GTX103558) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NMDAR2A antibody used in IHC (Paraffin sections) (IHC-P). GTX103558

GTX103558 IHC-P Image

NMDAR2A antibody detects GRIN2A protein at membrane on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
NMDAR2A antibody (GTX103558) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NMDAR2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103558

GTX103558 ICC/IF Image

NMDAR2A antibody detects NMDAR2A protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR2A stained by NMDAR2A antibody (GTX103558) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169