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NMDAR2B antibody [C2C3], C-term

Anti-NMDAR2B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109713
Anti-NMDAR2B antibody [C2C3], C-term used in Western Blot (WB). GTX109713
Anti-NMDAR2B antibody [C2C3], C-term used in Western Blot (WB). GTX109713

Cat. No. GTX109713

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NMDAR2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

166 kDa. ( Note )

Positive Control

HepG2 , mouse brain , rat brain

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human NMDAR2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950452

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate ionotropic receptor NMDA type subunit 2B , EIEE27 , GluN2B , MRD6 , NMDAR2B , NR2B , NR3 , hNR3

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein

Background

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NMDAR2B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109713

GTX109713 ICC/IF Image

NMDAR2B antibody [C2C3], C-term detects NMDAR2B protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR2B protein stained by NMDAR2B antibody [C2C3], C-term (GTX109713) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NMDAR2B antibody [C2C3], C-term used in Western Blot (WB). GTX109713

GTX109713 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX109713 diluted at 1:1000

Anti-NMDAR2B antibody [C2C3], C-term used in Western Blot (WB). GTX109713

GTX109713 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2B antibody [C2C3], C-term (GTX109713) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tan Y et al. Front Pharmacol 2020; 11 : 183 LY354740 Reduces Extracellular Glutamate Concentration, Inhibits Phosphorylation of Fyn/NMDARs, and Expression of PLK2/pS129 帢-Synuclein in Mice Treated With Acute or Sub-Acute MPTP.
Application : WB
Reactivity : Mouse
Algamal M et al. J Neurotrauma 2019; (Epub) Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169