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NMDAR2B antibody

Anti-NMDAR2B antibody used in IHC (Paraffin sections) (IHC-P). GTX130184
Anti-NMDAR2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130184
Anti-NMDAR2B antibody used in Western Blot (WB). GTX130184

Cat. No. GTX130184

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NMDAR2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

166 kDa. ( Note )

Positive Control

mouse hippocampus

Predict Reactivity

Human, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of rat NMDAR2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886203

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate ionotropic receptor NMDA type subunit 2B , GluN2B

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein

Background

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Database

Research Area

DATA IMAGES

Anti-NMDAR2B antibody used in IHC (Paraffin sections) (IHC-P). GTX130184

GTX130184 IHC-P Image

NR2B antibody detects NR2B protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NR2B antibody (GTX130184) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMDAR2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130184

GTX130184 ICC/IF Image

NMDAR2B antibody detects NMDAR2B protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR2B protein stained by NMDAR2B antibody (GTX130184) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NMDAR2B antibody used in Western Blot (WB). GTX130184

GTX130184 WB Image

NR2B antibody detects NR2B protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with NR2B antibody (GTX130184) at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169