GeneTex
United States (US)

NMDAR2B (phospho Tyr1472) antibody

Orthogonal
GTX31086_1525_WB_18121410_688.jpg

Cat No. GTX31086

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

166 kDa. ( Note )

Specificity/Sensitivity

Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either gamma-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
PROPERTIES

Form

Liquid

Buffer

10mM HEPES pH 7.5, 0.15M NaCl, 0.1 mg/ml BSA, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr1472 of NMDA NR2B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate Ionotropic Receptor Nmda Type Subunit 2B,Glun2B,Grin2B

Cellular Localization

Cell membrane, Multi-pass membrane protein,Cell junction,synapse,postsynaptic cell membrane, Multi-pass membrane protein

Background

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Database

Research Area

DATA IMAGES
GTX31086_1525_WB_18121410_688.jpg

GTX31086 WB Image

Orthogonal

WB analysis of rat hippocampal lysate using GTX31086 NMDAR2B (phospho Tyr1472) antibody.
Lane 1 : without lambda phosphatase
Lane 2 : lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min)

Package List Price ($)
$ 319