Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
Not tested in other applications.
Calculated MW
Product Note
Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either gamma-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Form
Liquid
Buffer
10mM HEPES pH7.5, 0.15M NaCl, 0.1mg/ml BSA, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr1472 of NMDA NR2B.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.