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NME4 antibody [4F10]

Anti-NME4 antibody [4F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84034
Anti-NME4 antibody [4F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84034
Anti-NME4 antibody [4F10] used in Flow cytometry (FACS). GTX84034
Anti-NME4 antibody [4F10] used in Western Blot (WB). GTX84034
Anti-NME4 antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX84034
Anti-NME4 antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX84034

Cat. No. GTX84034

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all NME4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NME4 (NP_005000) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 4 , NDPK-D , NM23H4 , nm23-H4

Cellular Localization

Mitochondrion matrix

Background

Major role in the synthesis of nucleoside triphosphates other than ATP.

Database

Research Area

DATA IMAGES

Anti-NME4 antibody [4F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84034

GTX84034 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NME4 plasmid using GTX84034 NME4 antibody [4F10].

Anti-NME4 antibody [4F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84034

GTX84034 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84034 NME4 antibody [4F10].

Anti-NME4 antibody [4F10] used in Flow cytometry (FACS). GTX84034

GTX84034 FACS Image

FACS analysis of HEK293T cells transfected with either NME4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84034 NME4 antibody [4F10].

Anti-NME4 antibody [4F10] used in Western Blot (WB). GTX84034

GTX84034 WB Image

WB analysis of HEK293T cells transfected with NME4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84034 NME4 antibody [4F10].
Loading : 5 ug per lane

Anti-NME4 antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX84034

GTX84034 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84034 NME4 antibody [4F10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NME4 antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX84034

GTX84034 IHC-P Image

IHC-P analysis of human colon tissue using GTX84034 NME4 antibody [4F10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519