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NMT1 antibody

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852
Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852
Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852
Anti-NMT1 antibody used in Western Blot (WB). GTX130852
Anti-NMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130852

Cat. No. GTX130852

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886365

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

N-myristoyltransferase 1 , NMT

Cellular Localization

Cytoplasm

Background

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852

GTX130852 IHC-P Image

NMT1 antibody detects NMT1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NMT1 antibody (GTX130852) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852

GTX130852 IHC-P Image

NMT1 antibody detects NMT1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NMT1 antibody (GTX130852) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852

GTX130852 IHC-P Image

NMT1 antibody detects NMT1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
NMT1 antibody (GTX130852) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMT1 antibody used in Western Blot (WB). GTX130852

GTX130852 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NMT1 antibody (GTX130852) diluted at 1:5000.

Anti-NMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130852

GTX130852 ICC/IF Image

NMT1 antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMT1 protein stained by NMT1 antibody (GTX130852) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169