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NMT1 antibody

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852
Anti-NMT1 antibody used in Western Blot (WB). GTX130852
Anti-NMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130852
Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852
Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852

Cat. No. GTX130852

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886365

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-myristoyltransferase 1 , NMT

Cellular Localization

Cytoplasm

Background

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852

GTX130852 IHC-P Image

NMT1 antibody detects NMT1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
NMT1 antibody (GTX130852) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMT1 antibody used in Western Blot (WB). GTX130852

GTX130852 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NMT1 antibody (GTX130852) diluted at 1:5000.

Anti-NMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130852

GTX130852 ICC/IF Image

NMT1 antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMT1 protein stained by NMT1 antibody (GTX130852) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852

GTX130852 IHC-P Image

NMT1 antibody detects NMT1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NMT1 antibody (GTX130852) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130852

GTX130852 IHC-P Image

NMT1 antibody detects NMT1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NMT1 antibody (GTX130852) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169