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NNT antibody

Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015
Anti-NNT antibody used in Western Blot (WB). GTX103015
Anti-NNT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103015
Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015
Anti-NNT antibody used in Western Blot (WB). GTX103015
Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015
Anti-NNT antibody used in Western Blot (WB). GTX103015

Cat No. GTX103015

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , A375 , rat kidney

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NNT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nicotinamide nucleotide transhydrogenase , GCCD4

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein; Matrix side

Background

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015

GTX103015 IHC-P Image

NNT antibody detects NNT protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NNT antibody (GTX103015) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NNT antibody used in Western Blot (WB). GTX103015

GTX103015 WB Image

NNT antibody detects NNT protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg HepG2 whole cell lysate/extract
| D. 30 µg A375 whole cell lysate/extract
7.5% SDS-PAGE
NNT antibody (GTX103015) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NNT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103015

GTX103015 ICC/IF Image

NNT antibody detects NNT protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NNT protein stained by NNT antibody (GTX103015) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015

GTX103015 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using NNT(GTX103015) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NNT antibody used in Western Blot (WB). GTX103015

GTX103015 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody (GTX103015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015

GTX103015 IHC-P Image

NNT antibody detects NNT protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
NNT antibody (GTX103015) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NNT antibody used in Western Blot (WB). GTX103015

GTX103015 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody (GTX103015) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169