GeneTex
United States (US)

NOD1 antibody [C2C3], C-term

GTX108811_IFA_19011507_452.jpg
GTX108811_WB_19011507_339.jpg

Cat No. GTX108811

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NOD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CARD4 antibody, CLR7.1 antibody, NLRC1 antibody, NOD1 antibody, "NLR family, CARD domain containing 1 antibody", "caspase recruitment domain family, member 4 antibody", caspase recruitment domain-containing protein 4 antibody, nucleotide-binding oligomerization domain-containing protein 1 antibody, "nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1 antibody", nucleotide-binding oligomerization domain containing 1 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX108811_IFA_19011507_452.jpg

GTX108811 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NOD1(GTX108811) antibody at 1:200 dilution.

GTX108811_WB_19011507_339.jpg

GTX108811 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX108811 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf