GeneTex
United States (US)

NOD2 antibody [2D9]

GTX30615_98_FACS_18121410_903.jpg
GTX30615_1425_WB_18121410_297.jpg
GTX30615_1427_WB_18121410_713.jpg
GTX30615_97_FACS_18121410_967.jpg
GTX30615_1428_WB_18121410_419.jpg
GTX30615_1426_WB_18121410_276.jpg
GTX30615_216_ICC-IF_18121410_789.jpg

Cat No. GTX30615

Host Mouse
Clonality Monoclonal
Clone Name 2D9
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100 - 1:2000
ICC/IF 1:50 - 1:500
IHC-P 1:10 - 1:500
FACS Assay dependent
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

115 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleotide Binding Oligomerization Domain Containing 2,Acug,Blau,Blaus,Card15,Cd,Clr16.3,Ibd1,Nlrc2,Nod2B,Psoras1,Yaos,Nod2

Cellular Localization

Cytoplasm

Background

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30615_98_FACS_18121410_903.jpg

GTX30615 FACS Image

FACS (Intracellular staining) analysis of THP-1 cells using GTX30615 NOD2 antibody [2D9].
Blue : Primary antibody
Orange : isotype control
Dilution : 5 μg/ml
Fixation : 4% PFA
Permibilization : 0.1% saponin

GTX30615_1425_WB_18121410_297.jpg

GTX30615 WB Image

WB analysis of HEK293 overexpressing NOD2 cell lysate using GTX30615 NOD2 antibody [2D9].

GTX30615_1427_WB_18121410_713.jpg

GTX30615 WB Image

WB analysis of THP-1 cell lysate using GTX30615 NOD2 antibody [2D9].
Dilution : 2 μg/ml

GTX30615_97_FACS_18121410_967.jpg

GTX30615 FACS Image

FACS (Intracellular staining) analysis of Raji cells using GTX30615 NOD2 antibody [2D9].
Blue : Primary antibody
Orange : isotype control
Dilution : 5 μg/ml
Fixation : 4% PFA
Permibilization : 0.1% saponin

GTX30615_1428_WB_18121410_419.jpg

GTX30615 WB Image

WB analysis of THP-1, RAW264.7, and U2OS cell lysate using GTX30615 NOD2 antibody [2D9].
Dilution : 2 μg/ml

GTX30615_1426_WB_18121410_276.jpg

GTX30615 WB Image

WB analysis of HT-29 cell lysate using GTX30615 NOD2 antibody [2D9].
Loading : 50μg

GTX30615_216_ICC-IF_18121410_789.jpg

GTX30615 ICC/IF Image

ICC/IF analysis of A431 cells using GTX30615 NOD2 antibody [2D9].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Package List Price ($)
$ 339