GeneTex
United States (US)

NOD2 antibody

GTX30694_588_IHC-P_18121410_732.jpg
GTX30694_1496_WB_18121410_478.jpg
GTX30694_252_ICC-IF_18121410_834.jpg

Cat No. GTX30694

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/ml
ICC/IF 1:50 - 1:100
IHC-P 1:250
Not tested in other applications.

Calculated MW

115 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human NOD2 (between residues 1000-1040). [UniProt# Q9HC29]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACUG antibody , BLAU antibody , BLAUS antibody , CARD15 antibody , CD antibody , CLR16.3 antibody , IBD1 antibody , NLRC2 antibody , NOD2B antibody , PSORAS1 antibody , YAOS antibody

Cellular Localization

Cytoplasm

Background

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30694_588_IHC-P_18121410_732.jpg

GTX30694 IHC-P Image

IHC-P analysis of human skin tissue using GTX30694 NOD2 antibody.
Dilution : 1:250
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

GTX30694_1496_WB_18121410_478.jpg

GTX30694 WB Image

WB analysis of HEK293 overexpressing NOD2 cell lysate using GTX30694 NOD2 antibody.
Loading : 20μg

GTX30694_252_ICC-IF_18121410_834.jpg

GTX30694 ICC/IF Image

ICC/IF analysis of HT-29 cells using GTX30694 NOD2 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

Package List Price ($)
$ 319