GeneTex
United States (US)

NOD2 antibody

GTX31664_2842_WB_20180221_18121410_192.jpg
GTX31664_259_ICC-IF_20180221_18121410_789.jpg
GTX31664_258_ICC-IF_20180221_18121410_709.jpg

Cat No. GTX31664

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899) , Human Lymph node Tissue Lysate(GTX29055)

Specificity/Sensitivity

At least four isoforms of NOD2 are known to exist; this antibody will only detect the longest isoform. NOD2 has no cross-reaction with NOD1.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NOD2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2.The immunogen is located within the first 50 amino acids of NOD2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleotide Binding Oligomerization Domain Containing 2,Acug,Blau,Blaus,Card15,Cd,Clr16.3,Ibd1,Nlrc2,Nod2B,Psoras1,Yaos,Nod2

Background

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31664_2842_WB_20180221_18121410_192.jpg

GTX31664 WB Image

WB analysis of human lymph node tissue lysate using GTX31664 NOD2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

GTX31664_259_ICC-IF_20180221_18121410_789.jpg

GTX31664 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31664 NOD2 antibody.
Working concentration : 20 μg/ml

GTX31664_258_ICC-IF_20180221_18121410_709.jpg

GTX31664 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31664 NOD2 antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329