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NOD4 antibody

Anti-NOD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85160
Anti-NOD4 antibody used in Western Blot (WB). GTX85160
Anti-NOD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85160

Cat. No. GTX85160

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all NOD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

205 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , EL4 Cell Lysate(GTX27183)

Product Note

NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NOD4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NOD4.The immunogen is located within amino acids 20 - 70 of NOD4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NLR family CARD domain containing 5 , CLR16.1 , NOD27 , NOD4

Cellular Localization

Cytoplasm

Background

NOD4 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD4 contains a CARD-like domain, a central NOD domain and a large LRR region. NOD4, an IFN-gamma-inducible nuclear protein, plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. As a key negative regulator of NF-ases, including cancer.

Database

Research Area

DATA IMAGES

Anti-NOD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85160

GTX85160 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85160 NOD4 antibody.
Working concentration : 10 μg/ml

Anti-NOD4 antibody used in Western Blot (WB). GTX85160

GTX85160 WB Image

WB analysis of EL4 cell lysate using GTX85160 NOD4 antibody.
Working concentration : 1 μg/ml

Anti-NOD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85160

GTX85160 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85160 NOD4 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Liang S et al. Exp Ther Med 2022; 24 (3) : 601 Inhibition of NLRC5 attenuates the malignant growth and enhances the sensitivity of gastric cancer cells to 5-FU chemotherapy by blocking the carcinogenic effect of YY1.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399