GeneTex
  • Country / Location Selection

United States (US)

NOL3 antibody

Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111883
Anti-NOL3 antibody used in Western Blot (WB). GTX111883
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111883

Cat No. GTX111883

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NOL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036255

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleolar protein 3 , ARC , FCM , MYP , NOP , NOP30

Cellular Localization

Isoform 1: Nucleus , nucleolus , Isoform 2: Cytoplasm

Background

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111883

GTX111883 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NOL3(GTX111883) antibody at 1:200 dilution.

Anti-NOL3 antibody used in Western Blot (WB). GTX111883

GTX111883 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX111883 diluted at 1:1000

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111883

GTX111883 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NOL3(GTX111883) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for NOL3 antibody (GTX111883). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NOL3 antibody (GTX111883). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169