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NOL3 antibody

Anti-NOL3 antibody used in Western Blot (WB). GTX113889
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889
Anti-NOL3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113889
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889
Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113889

Cat No. GTX113889

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168654

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleolar protein 3 , ARC , FCM , MYP , NOP , NOP30

Cellular Localization

Isoform 1: Nucleus , nucleolus , Isoform 2: Cytoplasm

Background

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOL3 antibody used in Western Blot (WB). GTX113889

GTX113889 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NOL3 antibody (GTX113889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOL3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113889

GTX113889 IHC-Fr Image

NOL3 antibody detects NOL3 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NOL3(GTX113889) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113889

GTX113889 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3(GTX113889) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169