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NOL3 antibody

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889
Anti-NOL3 antibody used in Western Blot (WB). GTX113889
Anti-NOL3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113889
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889
Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113889
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889
Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

Cat. No. GTX113889

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NOL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168654

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolar protein 3 , ARC , FCM , MYP , NOP , NOP30

Cellular Localization

Isoform 1: Nucleus , nucleolus , Isoform 2: Cytoplasm

Background

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOL3 antibody used in Western Blot (WB). GTX113889

GTX113889 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NOL3 antibody (GTX113889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOL3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113889

GTX113889 IHC-Fr Image

NOL3 antibody detects NOL3 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NOL3(GTX113889) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113889

GTX113889 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3(GTX113889) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

NOL3 antibody detects NOL3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113889

GTX113889 IHC-P Image

NOL3 antibody detects NOL3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NOL3 stained by NOL3 antibody (GTX113889) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zeng J et al. J Mol Neurosci 2018; (Epub) The Mechanism of Hyperalgesia and Anxiety Induced by Remifentanil: Phosphorylation of GluR1 Receptors in the Anterior Cingulate Cortex.
Application : IHC-Fr
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169