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NOL8 antibody

Anti-NOL8 antibody used in Immunoprecipitation (IP). GTX129790
Anti-NOL8 antibody used in Western Blot (WB). GTX129790

Cat No. GTX129790

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NOL8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleolar protein 8 , C9orf34 , NOP132 , bA62C3.3 , bA62C3.4

Cellular Localization

Nucleus , nucleolus

Background

NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 [PubMed 14660641]).[supplied by OMIM]

Database

DATA IMAGES

Anti-NOL8 antibody used in Immunoprecipitation (IP). GTX129790

GTX129790 IP Image

Immunoprecipitation of NOL8 protein from HeLa whole cell extracts using 5 μg of NOL8 antibody (GTX129790).
Western blot analysis was performed using NOL8 antibody (GTX129790).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NOL8 antibody used in Western Blot (WB). GTX129790

GTX129790 WB Image

NOL8 antibody detects NOL8 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with NOL8 antibody (GTX129790) diluted by 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169