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NOLC1 antibody

Anti-NOLC1 antibody used in Western Blot (WB). GTX115205
Anti-NOLC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115205
Anti-NOLC1 antibody used in Western Blot (WB). GTX115205

Cat. No. GTX115205

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NOLC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

H1299 , HCT116 , K562 , HL-60

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NOLC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885355

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolar and coiled-body phosphoprotein 1 , NOPP130 , NOPP140 , NS5ATP13 , P130

Cellular Localization

Nucleus , nucleolus , Cytoplasm

Background

Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.

Database

Research Area

DATA IMAGES

Anti-NOLC1 antibody used in Western Blot (WB). GTX115205

GTX115205 WB Image

NOLC1 antibody detects NOLC1 protein by western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg HL-60 whole cell lysate/extract
5 % SDS-PAGE
NOLC1 antibody (GTX115205) dilution: 1:5000

Anti-NOLC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115205

GTX115205 ICC/IF Image

NOLC1 antibody detects NOLC1 protein at cytoplasm and nucleolus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOLC1 protein stained by NOLC1 antibody (GTX115205) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NOLC1 antibody used in Western Blot (WB). GTX115205

GTX115205 WB Image

NOLC1 antibody detects NOLC1 protein by western blot analysis.
A. 30 μg H1299 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
5 % SDS-PAGE
NOLC1 antibody (GTX115205) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169